By Donald M. Kirschenbaum Ph. D. (auth.), Donald M. Kirschenbaum Ph. D. (eds.)
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Additional info for Atlas of Protein Spectra in the Ultraviolet and Visible Regions
O - 0 Casein. o 0 Gelatin. x x Ovalbumin. t::. t::. Protamine sulfate. 600 . 800 \ ' .. 400 . o\, '0 "b:xoooocxx>-o- o~ b '\ \ b '''' \_ 0.... ;. 40---2L60---28LO---:-3~OO::----:3~20:---::340 mlJ. 0 r - - - - - - , r - r - - - - - - , - - - - - - , il _ E. NAME: o. J. L. L. McMeekin, Arch. Biochem. , 93, 245 (1961). - ~ u z ~ )( COMMENTS: Curve A: as-Paracasein, rennin clot. LLI U milk Curve B: as-Casein. Curve C: al-Casein Curve D: Non-rennin-clotted portion of as-casein. 5 LL. U LLI a.. 02 N KOH.
0. (lJ u c o REFERENCE: R. Knippers and H. HoffmannBerling, J. Mol. , ll, 281 (1966) . 0 « COMMENTS: Protein concentration was 1 mg/ml. ---------------"""1 SOURCE: Pig brain EXPERIMENTAL CONDITIONS: Solvent- O. 8. 6 III REFERENCE: LoG. E. Kornguth, J. BioI. , 242, 4933 (1967). COMMENTS: Protein concentration was 2 mg/ml. 6. 2 N KOH. H. Ey1ar and M. Thompson, d c5 Arch. Biochem. , 129, 468 (1969). 0·8 COMMENTS: Protein concentration is 1 mg/m1. 225 250 275 300 325 WAVELENGTH (mJL) NAME: Basic Protein B2 SOURCE: Human plasma EXPERIMENTAL CONDITIONS: Solvent- REFERENCE: T.
G. P. Foust, J. Biol. , 244, 3999 (1969). 8, 17°C. REFERENCE: V. Massey, F. Muller, R. ~ r .... 1 , :,: \:\ \ " \ :: \ :\ \ 'I : \ \ \:\ \', OL---~----~ 350 400 ____L -____ 450 Wavelength (m,u) , \ .... ,,~. 3. Y. Miyake, K. Aki, S. Hashimoto, and T. Yamano, Biochim. Biophys. Acta, 105, 86 (1965). REFERENCE: (1) (1) o~--~~~==~==~~~~~~ 300 400 500 Wavelength (m .... ) NAME: Curve 1: Apoenzyme Curve 2: Holoenzyme Curve 3: Holoenzyme reduced with DL-alanine. 3 mg per ml in visible spectrum. 3. REFERENCE: K.