Fluorescence Studies on Biological Membranes by B. Wieb Van der Meer (auth.), H. J. Hilderson (eds.)

By B. Wieb Van der Meer (auth.), H. J. Hilderson (eds.)

As said via its first editor, Dr. D. B. Roodyn, the first target of the sequence Subcellular Biochemistry is to accomplish an built-in view of the mobile by means of bringing jointly effects from a variety of various innovations and disciplines. This quantity offers with the purposes of fluorescence spectroscopy to membrane learn. It seeks to give complementary biochemical and bio­ actual facts on either the constitution and the dynamics of organic membranes. Biophysics and biochemistry are enhancing a growing number of of their skill to check biomembranes, overlapping slightly during this region and explaining the functioning of the full mobilephone when it comes to the homes of its person com­ ponents. consequently, now we have introduced jointly a global team of specialists with the intention to record on and evaluation advances in fluorescence reviews on organic membranes, thereby highlighting subcellular points. the 1st chapters current a severe review of the present functions of dynamic and steady-state fluorescence innovations. next chapters dis­ cuss extra particular purposes in cells, organic membranes, and their con­ stituents (lipids, proteins).

Show description

Read Online or Download Fluorescence Studies on Biological Membranes PDF

Similar nonfiction_8 books

Crucial Issues in Semiconductor Materials and Processing Technologies

Semiconductors lie on the center of a few of an important industries and applied sciences of the 20 th century. The complexity of silicon built-in circuits is expanding significantly as a result of the non-stop dimensional shrinkage to enhance potency and performance. This evolution in layout ideas poses genuine demanding situations for the fabrics scientists and processing engineers.

3D Imaging in Medicine: Algorithms, Systems, Applications

The visualization of human anatomy for diagnostic, healing, and academic pur­ poses has lengthy been a problem for scientists and artists. In vivo scientific imaging couldn't be brought till the invention of X-rays through Wilhelm Conrad ROntgen in 1895. With the early scientific imaging innovations that are nonetheless in use this day, the 3-dimensional fact of the human physique can purely be visualized in two-dimensional projections or cross-sections.

Human Identification: The Use of DNA Markers

The continuing debate at the use of DNA profiles to spot perpetrators in legal investigations or fathers in paternity disputes has too frequently been carried out with out regard to sound statistical, genetic or criminal reasoning. The members to Human identity: The Use ofDNA Markers all have substantial event in forensic technological know-how, statistical genetics or jurimetrics, and plenty of of them have needed to clarify the clinical concerns excited by utilizing DNA profiles to judges and juries.

Vegetation and climate interactions in semi-arid regions

The chapters during this part position the issues of crops and weather interactions in semi-arid areas into the context which recur during the e-book. First, Verstraete and Schwartz overview desertification as a technique of international switch comparing either the human and climatic components. The subject of human influence and land administration is mentioned additional by means of Roberts whose evaluation specializes in semi-arid land-use making plans.

Extra resources for Fluorescence Studies on Biological Membranes

Sample text

A Fluorescence View on Membranes 35 Thomas and Stryer (1982) have applied diffusion-enhanced FET to elucidate the position of the retinal chromophore of rhodopsin in retinal disc membranes. 8 nm from the external surface of the disc membrane. 2 nm. However, factors such as irregularities of the membrane surfaces and electrostatic interactions can worsen the precision somewhat (Thomas and Stryer, 1982). Applications of diffusion-enhanced FET are also discussed by Yeh and Meares (1980), Meares and Rice (1981), and Isaacs et al.

Membrane asymmetry has been reviewed by Rothman and Lenard (1977), Op den Kamp (1979, 1981), and Storch and Kleinfeld (1985). The protein asymmetry is absolute; there is no transbilayer movement or «flip-flop" on proteins. Lipid flip-flop is slow; on the order of 8-16 hr at room temperature in erythrocytes and model membranes (Kornberg and McConnell, 1971; Seigneuret and Devaux, 1984). In erythrocytes, where the differences between inner and outer monolayers are known in detail, the aminophospholipids are located predominantly at the inside and phosphatidylcholine and sphingomyelin at the outside (Op den Kamp, 1979).

Clusters of phospholipids may exist at 15°C but are small compared to the patches at 5°C. Around 35°C the membrane is fluid and homogeneous. 2. Tryptophan Imaging of Membrane Proteins Determination of the molecular structure of membrane proteins is an important problem in molecular biology. Tertiary structure information originates most often from diffraction studies of crystalline proteins. Mapping of tryptophan residues can be achieved by FET between tryptophans and membrane probes (Kleinfeld, 1985).

Download PDF sample

Rated 4.82 of 5 – based on 37 votes