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I' ~- ;,/;1 ,, ,;~' 80 " ,~ " f'l 0 20 40 80 80 ~IMIDE(IIIM) FIGURE 5. Plots of the apparent pseudo first-order rate constants for the N-ethylmaleimide inhibition of the condensation reaction using 16:0 CoA (e), 6,9-18:2 CoA (0), and 6,9,12-18:3 CoA (~) as substrates. BIOSYNTHETIC PATHWAYS OF POLYUNSATURATED FATTY ACIDS 45 between the sl~pes for 6,9-18:2 CoA and 6,9,12-18:3 CoA was not significant (0 •. 2). 9-18:2 CoA or 6,9,12-18:3 CoA. The results are consistent with a common condensation pathway for both 6,9-18:2 CoA and 6,9,12-18:3 CoA.
BLANK, M. , PRIVETT, O. S. -o. (1964) J. Nutr. 83, 234-238. T. James Biosciences Division, Unilever Research Laboratory Colworth/Welwyn Colworth House, Sharnbrook, Bedford, England INTRODUCTION Studies of the metabolism of unsaturated fatty acids by mammals, particularly the rat, have been done over many years both by feeding unlabelled fatty acids followed by analytical and structural studies of the resultant tissue fatty acids and by using labelled substrates in whole animals, individual organs and tissue homogenates.
R. (1974) Mol. Cell Biochem. 1, 41-52. BUDNY, J. & SPRECHER, H. (1971) Biochim. Biophys. Acta 239, 190-207. DO, U. H. & SPRECHER, H. (1975) Arch. Biochem. 171, 597-603. DO, U. H. & SPRECHER, H. (1976) J. Lipid Res. 17, 424-430. , SHECHTER, I. & BLOCH, K. (1973) Science 182, 497-499. GURR, M. I. ) Vol. i, pp. 181-236, University Park Press, Baltimore. HILL, E. E. & LANDS, W. E. M. (1968) Biochim. Biophys. Acta 152, 645-648. HOLMAN, R. T. (1964) Federation Proc. 23, 1062-1067. KLENK, E. (1965) in Advances in Lipid Research (Paoletti, R.