By R. Jaenicke (auth.), U. B. Sleytr, P. Messner, D. Pum, M. Sára (eds.)
Immobilized useful biomolecules, relatively enzymes, are vital instruments in biotechnology, biochemistry, biochemical engineering, biomedicine and biosensor study. This booklet presents an creation and evaluation of chosen significant parts of the technological know-how and expertise of immobilized platforms. The chapters are meant as an advent and assessment to those interdisciplinary components, in addition to a resource of useful information and of recent examine developments. This ebook may be precious for scientists, technologists, lecturers and scholars in direct and similar fields.
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Additional info for Immobilised Macromolecules: Application Potentials
Other areas of future developments with immobilised macromolecules and cells include miniaturisation, particularly for analytical purposes. Other developments will focus on advances in support structure and preparation such as the use of continuous gels as well as the develpment of synthetic protein mimics through the imprinting technique (Hjertcn et al. 1989; Ekberg and Mosbach 1989). 33 Immobilisation of Macromolecules and Cells GROW CELLS MIX WITH POLYMER .. APPLY WASH AND FRACTIONATE COOLON ICE PARTITION TO AQUEOUS BUFFER Fig.
A general rule of thumb is that a pore size at least on the order of four to five times the maximum dimension of either the immobilised protein or cell should be chosen when they are immobilised via either adsorption or covalent coupling to a preformed porous support, or of the interacting partner. Of course, when the protein or cell is to be entrapped the pore size must be considerably smaller than the immobilised entity yet large enough to allow free passage of any compounds which are to interact with the immobilised protein or cell.
For instance, Sepharose activated with divinylsulphate is more suitable for the immobilisation of an antibody than Sepharose activated with BrCN if alkaline buffers are supposed to be used for antibody elution. (Lilme et al. 1986). 42 Immobilised Macromolecules: Application Potentials Oriented Immobilisation of Antibodies The immobilisation of antibodies via the Fc region of the heavy immunoglobulin chain seems to be very suitable because the antibody binding sites are located on the opposite part of the molecule.