Ion Interactions in Energy Transfer Biomembranes by Stuart McLaughlin (auth.), G. C. Papageorgiou, J. Barber, S.

By Stuart McLaughlin (auth.), G. C. Papageorgiou, J. Barber, S. Papa (eds.)

The professional Committee on Biomaterials and Biotechnology for the eu and the North American zone used to be based through the final meeting of UNESCO at its twenty first consultation, in 1981. The Committee contains a Coordinating staff and 4 operating teams, outlined within the following medical components: workforce I Proteins: resource, constitution and serve as. workforce II Nucl~ic acids: the hereditary fabrics. staff III Im~une fabrics and mechanisms. Membranes and delivery in biosystems. workforce IV In fulfilment of 1 of the pursuits of the Committee, which were followed by way of the overall meeting of UNESCO in 1981, specifically the intensification of the trade of clinical info on biomaterials and biotechnology, operating team IV equipped a global workshop on Ion Interactions in power delivery platforms, which was once convened in Athens, Greece, from eight to twelve April, 1985. clinical papers awarded at that workshop make up the chapters offered during this quantity. the current quantity focusses on traditional and synthetic membranes which are thinking about strength transduction. numerous chapters are dedicated to membranes and membrane elements that convert and make the most of gentle, resembling the thylakoid membrane of oxygenic photosynthetic organisms (eukaryotic and prokaryotic), the chromatophore membrane of nonoxygenic photosynthetic micro organism and the red membrane of the halophilic micro organism. different structures tested comprise the mitochondrial membranes and their adenine nucleotide service, the plasma membrane of animal cells, and lipid bilayer vesicles, both reconstituted or no longer, with enzymes.

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RESULTS ANS is a negatively charged fluorescent probe that has been used widely in mitochondria and other biological membrane (15-17). It is well known that energization of mi tochondria induce quenching of the probe fluorescence which was shown to result form a decrease of the binding constant (15). This was recently interpreted (10) as du~ to an increase of the surface charge. In non-energized mitochondria the fluoresence and binding of ANS indeed appear to correlate with the surface charge. Figure 1A shows a titration of the fluorescence with salts.

Q " '0. DD "- - -- - - - - - ~ - - - ~'- I o~ '. 0 10· FIGURE 1: Salt dependence of ANS fluorescence and the calculated surface potential (Robertson and Rottenberg ref. 18). 25 M sucrose) as function of NaCl (0), KCl (I) MgS0 4 (A) and CaP04 (A). B: Null-point titration for 0 determination. ~ of 250 p'M Cj:C12' C: Effect of Na+ (I), K+ (~) Mg + (0), Ca +(0) ana La + (A) on the calculated 'f's' The solid lines are calculated from the Gouy-Chapman theory (see text). 't' If the fluorescence quenching observed on energization is due to increased surface charge, screening with salts should enhance the fluorescence to its original, non-energized value.

06 N=5 = ITP = 5 pM. Cat 9 =50)lM Table 1 presents the results of two experiments. 50 In the first, the absolute surface potentials of untreated and FCCP treated mitochondria were compared. Note that, although the difference in absolute potentials is -9 millivolts, the relative potential difference under low salt conditions is -15 millivolts. values of P. This discrepancy is due to the difference in the high salt In the second experiment, the relative surface potential dif- ference between ATP- and (ITP+FCCP)-treated mitochondria was found to be -14 millivolts.

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