By John R. Beaman Jr., Jerry L. Campbell
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Additional info for Messerschmitt Bf 109 Part 1 in Action No 44
Unfortunately, none of them are effective for immunopurification of phytochrome as weIl. Pea-25, for example, will not immunopurify the pea phytochrome to which it is directed, even after the phytochrome has been mildly denatured (unpublished observations). It is therefore impractical with present antibodies to immunopurify 123-kDa green-oat phytochrome. Relative abundance 0/ etiolated-oat phytochrome in green oat leaves Of the seven MAbs to green-oat phytochrome, only one (GO-1) cross reacts with etiolated-oat phytochrome.
SDLESIIE .. ASLQSIED .. MDLESIED .. SDIEGIEE .. GSVEISC AG. GQLTIST SG. • TDTMLFPF .. •.. TAELAAAPSA VGR ••••••. •. •..... \ 6A di\ ill6~ & :nNMI 100 200 300 400 500 AMINO 600 ACID 700 800 900 1000 A 1100 RESIDUES COOH Figure 3. (Top) Hydropathy profiles of Arabidopsis phyA, phyB, and phyC (modified after Distribution of invariant amino acid residues Sharrock and Quail, 1989). (Bottom) along the aligned polypeptides of all phy sequences currently available (see Fig. 2). The number of invariant residues (consensus line in Fig.
Since the phyB and phyC sequences have 2 and 3 substitutions, respectively, it will be of interest to determine whether P-25 is capable of recognizing either of these polypeptides. 30 PHYTOCHROME-DEFICIENT MUTANTS AND OVEREXPRESSION IN TRANSGENIC PLANTS The question of the potential functional significance of various regions and sequence motifs in the phytochrome polypeptide can be addressed using targeted in vitro mutagenesis of phy sequences followed by examination of the effects of the mutagenesis in transgenic plants.